Antioxidant and hepatoprotective effect of the roots of Hibiscus esculentus Linn.

J. Anbu Jeba Sunilson, P. Jayaraj, M. Syam Mohan, A. Anita Gnana Kumari, R. Varatharajan

Abstract


Th e ethanol extract of Hibiscus esculentus Linn roots inhibited the formation of oxygen derived free radicals invitro with IC50 of
620 μg/ml, 2300 μg/ml, 870 μg/ml and 610 μg/ml. [Fe2+/ascobate system], 730 μg/ml, [Fe3+/ADP/ascorbate system], for superoxide radical production, hydroxyl radical generation, nitric oxide radical formation and lipid peroxide formation respectively. Th e oral administration of the extract (250 and 500 mg/kg body weight), signifi cantly reduce CCl4 induced hepatotoxicity in rats, as judged from the serum and tissue activity of marker enzymes [Glutamate oxaloacetate transaminase (GOT), Glutamate pyruvate transaminase
(GPT) and alkaline phosphatase (ALP)]. Th ese results were comparable with standard drug silymarin [20 mg/kg, P.O.)
Key words: Hibiscus esculentus Linn, ethanolic extract, antioxidant activity, hepatoprotective eff ect

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References


WHO, Hepatitis- B (online) 2000. Available from: htt p://www.who.

int/entity/mediacentre. [last accessed on2006 Dec 11].

Sunitha S, Nagaraj M, Varalakshmi P. Hepatoprotective eff ect of

lupeol linoleate on tissue antioxidant defense system in cadmiuminduced

hepatotoxicity in rats. Fitoterapia 2001;72:512-23.

Osawa T, Kayakishi S, Namiki M. Antimutagenesis and

anticarcinogenesis mechanisms. New York: Plenum Ltd; 1990.

p. 139-53.

Harborne JB. Phytochemical methods. London: Chapman and

Hall Ltd; 1996. p. 52-105.

Liao K, Yin M. Individual and combined antioxidant eff ects of

seven phenolic agents in human erythrocyte membrane ghosts

and phosphatidylcholine liposome systems: Importance of the

partition coeffi cient. J Agric Food Chem 2000;48:2266-70.

Indian medicinal plants. Hyderabad, India: Orient Longman

Limited; 1994. p. 1-3.

Barrett B. Medicinal plants of Nicoraguas Atlantic Coast. Econ

Bot 1994;481:8-20.

Yesilada E, Honda G, Sezik E, Tabata M, Fusita T, Takenda Y, et al.

Traditional Medicine in Turkey, Folk medicine in the inner Taurus

mountain. J Ethano Pharmacol 1995;463:133-52.

Tomoda M, Shimizu N, OshimaY, Takahashi M, Murakami M,

Hikino H. Hypoglycaemic activity of twenty plant mucilages and

three modiÞ ed products-1. Planta Med 1987;531:8-12.

Carini R, Comoglio A, Albano E, Poli G. Lipid peroxidation and

irreversible damage in the rat hepatocyte model: Protection by

the silybin-phospholipid complex IdB 1016. Biochem Pharmacol

;43:2111-5.

Manna SK, Mukhopadhyay A, Van NT, Aggarwal BB. Silymarin

suppresses TNF-induced activation of NF-jB, c-Jun Nterminal

kinase, and apoptosis. J Immunol 1999;163:6800-9.

Skott ova N, Kazdova L, Oliyarnyk O, Vecera R, Sobolova L,

Ulrichova J. Phenolics-rich extracts from Silybum marianum and

Prunella vulgaris reduce a high-sucrose diet induced oxidative

stress in hereditary hypertriglyceridemic rats. Pharmacol Res

;50:123-30.

Trease GE, Evans WC. Pharmacogonosy. London: W.O.Soundars

Company Ltd.; 1996. p. 251.

McCord JM, Fridovich I. Superoxide dismutase: An enzymatic

function of Erythocupin (nemocupin). J Biol Chem 1969;224:

-55.

Elizabeth K, Rao MN. Oxygen scavenging activity of curcumin,

Int Pharm 1990;58:37.

Ohkawa H, Ohishi N, Yagi K. Assay of lipid peroxidase in

animal tissues by thiobarbituric acid reaction. Anal Biochem

;95:351-8.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS,

Tannenbaum SR. Analysis of nitrate, nitrite and 15[N] Nitrate in

biological ß uids. Anal Biochem 1982;1216:131.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Parker L. The nitric

oxide scavenging eff ect of Ginko biloba extract EGb 761. Biochem

Biophysys Res Commun 1994;201:748-55.

Biyashee S, Balasubramanian AS. Annual breeding cycle

and spawning behavior of Hyla annectans. J Neurochem

;18:990.

Nishigaki I, Kuttan R, Oku H, Ashoori F, Abe H, Yagi K.

Suppressive eff ect of curcumin of lipid peroxidase induced in rats

by carbon tetra chloride or cobalt-60 irradiation. J Clin Biochem

Nutr 1992;13:23-9.

Kind PR, King EJ. Method of practical clinical biochemistry. In:

Invarley H, Gowenlock AH, editors. London: Bell Meditors; 1980.

p. 899-900.

Reitman S, Frankel S. A Colourimetric method for the determination

of serum glutamic oxalacetic and glutamic pyruvic transaminases.

Am J Clin Pathol 1957;28:56-63.

Kulkarni SK. Hand book of experimental pharmacology. New

Delhi, India: Vallabh Prakashan; 1999. p. 172.

Di Carlo G, Mascolo N, Izzo AA. Capasso F. Flavonoids: Old

and new aspects of a class of natural therapeutic drugs. Life Sci

;65:337-53.

Oh H, Kim DH, Cho JH, Kim YC. Hepatoprotective and free radical

scavenging activities of phenolic petrosins and ß avonoids isolated

from Equisetum arvense. J Ethnopharmacol 2004;95:421-4.




DOI: http://dx.doi.org/10.22377/ijgp.v2i4.3

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