Neuro beneficial effects of Pimpinella anisum against lead exposure

Khaled Kahloula, Miloud Slimani, Djallel Eddine Houari Adli, Sahra Rachdi, Dallel Boumediene

Abstract


Background: The essential oil of Pimpinella anisum has been widely used in traditional medicine to treat a variety of diseases,
including some neurological disorders. Aims: This study was aimed to test, in vivo, the possible anxiolytic and antidepressant
effects, of the essential oil of Pimpinella anisum against chronic lead acetate (0.2%) intoxication during the gestation and lactation period, in Wistar rat pups. Settings and Design: Wistar rat pups were exposed to lead via their dams’ drinking water from postnatal day (PND) 1 to (PND) 21. After weaning, the lead‑exposed rats received injections of essential oil of Pimpinella anisum (0.5 ml/kg) for 15 days. The level of anxiety, depression and locomotor activity were studied. Materials and Methods: The behaviours evaluated were: Locomotor activity (open‑field test), anxiety (dark and light compartment and elevated plus maze tests), and depression (forced
swimming test). Statistical Analysis: The data were analysed by two‑way analyses of variance (ANOVAs). When a significant
difference was found, the Student‑Newman‑Keuls post‑hoc test was conducted. For all analyses, the difference was considered to
be significant at P ≤ 0.05. Results: The results of the present study demonstrate that developmental lead exposure induces, on the one hand, impairments of body (P < 0.001) and brain weight (P < 0.05), respectively, and on the other hand, increases the level of anxiety (P < 0.001), depression (P < 0.001) and locomotor hyperactivity (P < 0.001), compared to control rats. Administration of the essential oil of Pimpinella anisum entrains reduction in the level of anxiety (P < 0.001), depression (P < 0.001) and correct locomotor hyperactivity (P < 0.001) in rats exposed to lead beforehand. Conclusion: In conclusion, our results demonstrate that developmental lead exposure induces significant perturbation of emotional reactivity that can be improved by treatment with the essential oil of
Pimpinella anisum. Further evaluation of the use of anise oil in the treatment of neurological disorders is suggested.
Key words: Anxiety, depression, lead acetate, locomotor activity, Pimpinella anisum

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Shalan MG, Mostafa MS, Hassouna MM, El‑Nabi SE, El‑Refaie A.

Amelioration of lead toxicity on rat liver with Vitamin C and

silymarin supplements. Toxicology 2005;206:1‑15.

Bourljeily N, Suszkiw JB. Developmental cholinotoxicity of

lead: Loss of septal cholinergic neurons and long‑term changes

in cholinergic innervation of the hippocampus in perinatally

lead‑exposed rats. Brain Res 1997;771:319‑28.

Murphy KJ, Regan CM. Low level lead exposure in the

early postnatal period results in persisting neuroplastic

deficits associated with memory consolidation. J Neurochem

;72:2099‑104.

Finkelstein Y, Markowitz ME, Rosen JF. Low‑level lead‑induced

neurotoxicity in children: An update on central nervous system

effects. Brain Res Rev 1998;27:168‑76.

Moreira EG, Vassilieff I, Vassilieff VS. Developmental lead

exposure: Behavioural alterations in the short and long term.

Neurotoxicol Teratol 2001;23:489‑95.

Kuhlmann AC, Mc Glothan JL, Guilarte TR. Developmental lead

exposure causes spatial learning deficits in adult rats. Neurosci

Lett 1997;233:101‑4.

Silbergeld EK. Mechanisms of lead neurotoxicity, or looking

beyond the lamppost. FASEB J 1992;6:3201‑6.

Winneke G, Lilienthal H, Krämer U. The neurobehavioural

toxicology and teratology of lead. Arch Toxicol Suppl 1996;

:57‑70.

Lanphear BP, Dietrich K, Auinger P, Cox C. Cognitive deficits

associated with blood lead concentrations <10 microg/dl in US

children and adolescents. Public Health Rep 2000;115:521‑9.

Gilbert ME, Mack CM, Lasley SM. Chronic developmental lead

exposure increases the threshold for long‑term potentiation in rat

dentate gyrus in vivo. Brain Res 1996;736:118‑24.

Jett DA, Kuhlmann AC, Farmer SJ, Guilarte TR. Age‑dependent

effects of developmental lead exposure on performance in the

Morris water maze. Pharmacol Biochem Behav 1997;57:271‑9.

Toscano CD, Hashemzadeh‑Gargari H, Mc Glothan JL, Guilarte TR.

Developmental Pb2+exposure alters NMDAR subtypes and

reduces CREB phosphorylation in the rat brain. Dev Brain Res

;139:217‑26.

Schneider JS, Huang FN, Vemuri MC. Effects of low‑level

lead exposure on cell survival and neurite length in primary

mesencephalic cultures. Neurotoxicol Teratol 2003;2:555‑9.

Toscano CD, McGlothan JL, Guilarte TR. Lead exposure alters

cyclic‑AMP response element binding protein phosphorylation

and binding activity in the developing rat brain. Brain Res Dev

Brain Res 2003;145:219‑28.

Cory‑Slechta DA. Relationships between lead‑induced learning

impairments and changes in dopaminergic, cholinergic, and

glutamatergic neurotransmitter system functions. Annu Rev

Pharmacol Toxicol 1995;35:391‑415.

Steflitsch W, Steflitsch M. Clinical aromatherapy. J Mens Health

;5:74-85.

Simon J, Chadwick AH. An Indexed Bibliography 1971–1980

the Scientific Literature on Selected Herbs, and Aromatic and

Medicinal Plants of the Temperate. In: Craker LE, editor. Hamden:

Zone Archon Books; 1984. p. 770.

Gorji A, Khaleghi GM. History of epilepsy in Medieval Iranian

medicine. Neurosci Biobehav Rev 2001;25:455‑61.

Costall B, Domeney A, Gerrard MP, Kelly AM, Naylor ER.

Zacopride: Anxiolytic profile in rodent and primate models of

anxiety. J Pharma Pharmacol 1988;40:302‑5.

Porsolt RD, Le Pichon M, Jalfre M. Depression: A new animal model

sensitive to antidepressant treatments. Nature 1977;266:730‑2.

Dauge V, Rossignol P, Roques BP. Comparison of the behavioural

effects induced by administration in rat nucleus accumbens

or nucleus caudatus of selective mu and delta opioid peptides

or kelatorphan, an inhibitor of enkephalin metabolism.

Psychopharmaco 1989;96:343‑52.

Pellow S, Chopin P, File SE, Briley M. Validation of open‑closed

arm entries in an elevated plus‑maze as a measure of anxiety in

the rat. J Neurosci Methods 1985;14:149‑67.

Alfano DP, LeboutillIer JC, Petit TL. Hippocampal mossy fiber

pathway development in normal and postnatally lead‑exposed

rats. Exp Neurol 1982;75:308‑19.

Cory‑Slechta DA, Widzowski DV. Low level lead exposure

increases sensitivity to the stimulus properties of dopamine D1

and D2 agonists. Brain Res 1991;553:65‑74.

Gilbert ME, Mack CM, Lasley SM. The influence of developmental

period of lead exposure on long‑term potentiation in the adult rat

dentate gyrus in vivo. Neurotoxicol 1999;20:57‑69.

Kiraly E, Jones DG. Dendritic spine changes in rat hippocampal

pyramidal cells after postnatal lead treatment: A golgi study. Exp

Neurol 1982;77:236‑9.

Yang Y, Ma Y, Ni L, Zhao S, Li L, Zhang J. Lead exposure through

gestation‑only caused long‑term learning/memory deficits in

young adult offspring. Exp Neurol 2003;184:489‑95.

Bellinger DC. Effect modification in epidemiological studies of

low level neurotoxicant exposures and health outcomes. Neurotox

Teratol 2000;22:133‑40.

Koch C, Reichling J, Schneele J, Schnitzler P. Inhibitory effect of

essential oils against herpes simplex virus type 2. Phytomedicine

;15:71‑8.

Waumans D, Bruneel N, Tytgat J. Anise oil as paramethoxyamphetamine

(PMA) precursor. Forensic Sci Int

;133:159‑70.

Harris RB, Zhou J, Youngblood BD, Rybkin I, Smagin GN,

Ryan DH. Effect of repeated stress on body weight and body

composition of rats fed low‑ and high‑fat diets. Am J Physiol Regul

Integr Comp Physiol 1998;275:1928‑38.

Krigman MR, Hogen EL. Effects of Pb intoxication on the

postanatal growth of the rat nervous system. Environ Health

Perspect 1974;7:187‑99.

Gonz Z, Evans HL. Effects of chelation with meso‑dimercapto

succinic acid (DMSA) beforeand after the appearance of Pb

induced neurotoxicity in the rat. Toxicol Appl Pharmacol

;144:205‑14.

Press M. Neuronal development in the cerebellum of lead‑poisoned

neonatal rats. Acta Neuropathol 1977;40:259‑68.

Lampert PW, Garro F, Pentshew A. Lead encepholopathy

in suckling rats: An electron microscopic study. In: Klatzo I,

Seitalberges F, editors. Symposium on Brain Edema, Vienna:

Springer; 1967. p. 207.

Lorton D, Anderson WJ. The effects of postnatal lead toxicity on

the development of the cerebellum in rats. Neurochem Toxicol

Terato 1984;8:51‑9.

Voig JP, Rex A, Shor R, Fink H. Hippocampal 5‑HT and NE

release in the transgenic rat TGR (mREN2) related to behaviour

on the elevated plus‑maze. Eur Neuropsychopharmacol 1998;

:279‑85.

Antonio MT, Leret ML. Study of the neurochemical alterations

produced in discrete brain areas by perinatal low‑level lead

exposure. Life Sci 2000;67:635‑42.

De Souza Lisboa SF, Gonçalves G, Komatsu F, Queiroz CA,

Almeida AA, Moeraira EG. Developmental lead exposure induces

depressive‑like behaviour in female rats. Drug Chem Toxicol

;28:67‑77.

Estrada‑Camarena E, Fernandez Guasti A, Lopez‑Rubal

Cava C. Participation of the 5HT1A receptor in the

antidepressant‑like effect of estrogens in the forced swimming

test. Neuropsychopharmacology 2006;31:247‑55.

Leret ML, Antonio JSM, Antonio MT. Perinatal exposure to

lead and cadmium affects anxiety‑like behaviour. Toxicology

;186:125‑30.

Kasdallah AG, Mornaguib B, Gharbi N, Machgoul S, El‑Fazâa S.

Metabolic and endocrine effects of water and/or food deprivation

in rats. C R Biol 2005;328:463‑70.

Tzschentke TM. Glutamatergic mechanisms in different disease

states: Overview and therapeutical implications an introduction.

Amino Acids 2002;23:147‑52.

Poleszak E, Wlaź P, Kędzierska E, Nieoczym D, Wróbel A,

Fidecka S, et al. NMDA/glutamate mechanism of antidepressant‑like

action of magnesium in forced swim test in mice. Pharmacol

Biochemist Behav 2007;88:158‑64.

Foster AC, Kemp JA. Neurobiology. Glycine maintains excitement.

Nature 1989;338:377‑8.

Rodrigues AL, Rocha JB, Mello CF, Souza DO. Effect of perinatal

lead exposure on rat behaviour in open‑field and two‑way

avoidance tasks Pharmacol Toxicol 1996;79:150‑6.

Eppright TD, Sanfacon JA, Horwitz EA. Attention deficit

hyperactivity disorder, infantile autism, and elevated blood‑lead:

A possible relationship. Mo Med 1996;93:136‑8.

Sahraei H, Ghoshooni H, Hossein Salimi S, Mohseni Astani A,

Shafaghi B, Falahi M. The effects of fruit essential oil of the

Pimpinella anisum on acquisition and expression of morphine

induced conditioned place preference in mice. J Ethnopharmacol

;80:43‑7.

Kreydiyyeh SI, Usta J, Knio K, Markossian S, Dagher S. Aniseed

oil increases glucose absorption and reduces urine output in the

rat. Life Sci 2003;74:663‑73.

Karimzadeh F, Hosseini M, Mangeng D, Alavi H, Hassanzadeh GR,

Bayat M, et al. Anticonvulsant and neuroprotective effects of

Pimpinella anisum in rat brain. BMC Complement Altern Med

;12:76.




DOI: http://dx.doi.org/10.22377/ijgp.v7i1.290

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