Regeneration of pancreatic b‑cells on streptozotocin induced diabetic rats under the effect of Swietenia macrophylla seeds

Moumita Dutta, Utpal Kumar Biswas, Runu Chakraborty, Piyasa Banerjee, Utpal Raychaudhuri


Background: Diabetes mellitus is one of the major killers these days and has a profound effect in the quality of life. Despite considerable progress in the treatment of diabetes, search for newer drugs continues, especially from the natural resources. We have previously reported that the seed of the plant. Swietenia macrophylla is nontoxic. Aim: The current study was undertaken to investigate the effect of Swietenia macrophylla seeds on histopathological changes of pancreatic, hepatic and renal tissues, besides to observe its effect on the blood glucose level and lipid parameters in experimental diabetic rats. Materials and Methods: The Albino rat (Wister Strain) weighing 200‑250 gm were divided into five groups of seven rats each and streptozotocin was used to induce diabetes except
in the control group. The control group (I) and diabetic control group (II) received redistilled water. Group III and V diabetic rats
were administered with aqueous extract of Swietenia macrophylla seeds (2 gm/kg body weight) daily for 30 days. Peptide was mixed with extract as adjuvant in the latter group (1:1, 2 gm/kg body weight/day for 30 days). Group IV diabetic rats were fed with aqueous extract of standard drug Metformin (10 mg/kg body weight). Statistical Analysis: Statistical analysis was done using Microsoft Excel, Office 2003 and SPSS 16 software. Values were expressed by mean ± SD. P value <0.05, <0.01 were considered statistically significant.
Results: The oral administration of extract alone and extract plus soya peptide to diabetic rats was found to possess significant
hypoglycaemic as well as hypolipidemic activity. Histopathological changes include hypercellularity of islets tissue with increased
hyperchromic nucleus in the same group. Increased density of islets in pancreatic tissue with higher percent of b cells and islets size in this group, in comparison to diabetic untreated group indicate regeneration of beta cells under the effect of Swietenia macrophylla seeds, without any significant change in hepatic and renal tissues. Conclusion: We conclude that the Swietenia macrophylla seeds have potentials for development into a therapeutic agent in diabetes mellitus.
Key words: Diabetes mellitus, histopathology, lipid profile, Swietenia macrophylla seeds

Full Text:



Bhat M, Zinjarde SS, Bhargava SY, Kumar AR, Joshi BN. Antidiabetic

Indian plants: a good source of potent amylase inhibitors. Evid

Based Complement Alternat Med 2011;2011:810207.

Meenu J, Sharma S, Kumar M. Ameliorative potential of

vignamunga seeds on hyperglycaemia mediated oxidative stress

and hyperlipidemia in STZ diabetic rats. Int J Green Pharm


Sultana I, Noor MA, Barua J, Mahmood A, Das MC, Islam MT,

et al. In vitro anti‑antherothrombosis activity of four Bangladeshi

plants. Int J Green Pharm 2012;6:5‑8.

Maiti A, Dewanjee S, Mandal SC. In vivo evaluation of

antidiarrhoeal activity of the seed of Swietenia macrophylla king

(Meliaceae). Trop J Pharm Res 2007;6:711‑6.

Dutta M, Raychaudhuri U, Chakraborty R, Maji D. Role of diet

and plants on diabetic patients: A critical appraisal. Sci Cult


Sahgal G, Ramanathan S, Sasidharan S, Mordi MN, Ismail S,

Mansor SM. In vitro antioxidant and xanthine oxidase inhibitory

activities of methanolic Swietenia mahagoni seed extracts.

Molecules 2009;14:4476‑85.

Dutta M, Raychaudhuri U, Chakraborty R, Maji D. Sub‑chronic

toxicity study of the seeds of Swietenia macrophylla in wister rats.

Sci Cult 2012;78:78‑83.

Maiti A, Dewanjee S, Sahu R. Isolation of hypoglycaemic

phytoconstituent from Swietenia macrophylla seeds. Phytother Res


Public Health Service Policy on Human Care and Use of laboratory

animals. Washington, DC: US, Department of Health and Human

Services; 1986. p. 20892. Available from:

grants/olaw/olaw.htm [Last accessed on 2011 Jan 24].

Abeeleh MA, Ismail ZB, Alzaben KR, Abu‑Halaweh SA,

Al‑Essa MK, Abuabeeleh J, et al. Induction of diabetes mellitus in

rats using intraperitoneal streptozotocin: A comparison between

strains of rats. Euro J Sc Res 2009;32:398‑402.

Buzzetti R, Cernea S, Petrone A, Capizzi M, Spoletini M,

Zampetti S, et al. C‑peptide response and HLA genotypes in

subjects with recent‑onset type 1 diabetes after immunotherapy

with Dia Pep 277: An exploratory study. Diabetes 2011;60:3067‑72.

Awe S, Olayinka ET. Effect of cashew wine on histology and

enzyme activities of rat liver. J Toxicol and Environ Health Sc


Kumar A, Ilavarasan R, Jayachandran T, Deecaraman M,

Aravindan P, Padmanabhan N, et al. Anti‑diabetic activity

of Syzygiumcumini and its isolated compound against

streptozotocin‑induced diabetic rats. J Med Plants Res 2008;2:246‑9.

Rao MU, Sreenivasulu M, Chengaiah B, Reddy KJ, Chetty CM.

Herbal medicines for diabetes mellitus: A review. Int J Pharm Tech

Res 2010;2:1883‑92.

Kumar SK, Bhowmik D, Chiranjib, Biswajit. Aloe vera:

A potential herb and its medicinal importance. J Chem Pharm

Res 2010;2:21‑9.



  • There are currently no refbacks.