Comparative study of antitoxin activity of Calotropis gigentea Linn and Cassia fistula Linn against Naja-naja (cobra) venom

Surabhi Pandey, Emmanuel Toppo, Preeti Chauhan


Calotropis gigantea Linn and Cassia fistula Linn is used traditionally in India to treat snake bite patients. Hydroalcoholic extract of
dried leaves of Calotropis gigantea Linn and Cassia fistula Linn was tested for haemolysis, procoagulant, oedema-forming activity
against cobra (Naja-Naja) venom. The hydroalcoholic extract showed a significant inhibitory effect on haemolysis, procoagulant,
oedema-forming activity. Calotropis gigantea Linn extract was significantly neutralize Naja-naja venom as compared to Cassia fistula Linn in a dose dependent manner. The present study suggests that hydroalcoholic extract of dried leaves of Calotropis gigantea Linn and Cassia fistula Linn possess compounds, which neutralize the activity of cobra (Naja-Naja) venoms.
Key words: Oedema forming, hemolysis, Naja-naja, procoagulant

Full Text:



Ahmed A, Rajendaran K, Jaiswal D, Singh H, Mishra A, Chandra D,

et al. Anti-snake venom activity of different of Pouzolzia indica

against Russel viper venom. Int J ChemTech Res 2010;2:744-51.

Gomes A, Das R, Sarkhel S, Mishra R, Mukherjee S, Bhattacharya S,

et al. Herbs and herbal constituents active against snake bite. Indian

J Exp Biol 2010;48:865-78.

Meenatchisundaram S, Priyagrace S, Vijayaraghavan R,

Ambikapathi V, Parameswari G, Michael A. Antitoxin activity of

Mimosa pudica root extract against Naja-naja and Bangarus caerulus

venoms. J Bangladesh Pharmacol Soc 2009;4:105-9.

Meenatchisundaram S, Parameswari G, Michael A. Studies on

antivenom activity of Andrographis paniculata and Aristolochia indica

plant extracts against Daboia russelli venom by in vivo and in vitro

methods. Int J Sci Technol 2009;2:76-9.

Das S, Das S, Das MK, Basu SP. Evaluation of anti-inflammatory

effect of Calotropis gigentea and Tridex procumbens on wister albino

rats. J Pharm Sci Res 2009;14:123-6.

Vasudevan DT, Dinesh KR, Gopalakrishnan S, Sreekanth SK,

Shekar S. The potential of aqueous and isolated fraction from

leaves of Cassia fistula Linn as antibacterial agent. Int J Chem Sci


Rajendran K, Shirwaikar A, Mehta M, Bharathi RV. In vitro and

in vivo anti-snake venom (Daboia russelli) studies on various

leaf extracts of Acalypha indica Linn. Int J Phytomedicine 2010;2:


Igbe I, Ching F, Eromen A. Anti-iflammatory activity of aqueous

fruit pulp extract of Hunteria umbellate K. schum in acute and

chronic inflammation. Acta Pol Pharm Drug Res 2010;67:81-5.

Meenatchisundaram S, Michael A. Antitoxin activity of Mucuna

puriens aqueous extract against cobra and krait venom by in vivo

and in vitro methods. Int J Pharmatech Res 2010;2;870-4.



  • There are currently no refbacks.