Protective effect of speman on cisplatin-induced testicular and epididymal toxicity in mice

S. B. Sainath, T. Sowbhagyamma, P. Madhu, K. Pratap Reddy, B. P. Girish, P. Sreenivasula Reddy

Abstract


Testicular cancer is the most common cancer affecting men of reproductive age. Advances in treatment of the disease, which includes the administration of cisplatin, have brought the 5-year survival rate to over 90%. This high cure rate, coupled with young age of patients, makes elucidation of the impact of the treatment on reproduction become increasingly important. The objective of the present study was to investigate the protective effect of speman, a non-hormonal herbal formulation, on cisplatin-induced suppressed male reproductive health in mice. Male mice were treated with cisplatin or speman alone or in combination and assessed for spermatogenesis
and steroidogenesis. Significant decrease in the weights of testes and epididymis was observed in cisplatin treated animals. Injection of cisplatin significantly decreased epididymal sperm count, viable sperms, motile sperms and hypo-osmotic swelling (HOS)-tail coiled sperms with a significant reduction in the testicular steroidogenic enzyme activities and serum testosterone levels, whereas co-administration of speman with cisplatin showed a significant improvement in the selected reproductive parameters over cisplatin alone treated mice indicating the beneficial effect of speman to combat cisplatin-induced suppressed reproduction in male mice.
Key words: Cisplatin, male mice, speman, sperm, steroidogenesis

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References


Bergström R, Adami HO, Möhner M, Zatonski W, Storm H,

Ekbom A, et al. Increase in testicular cancer incidence in six

European countries: A birth cohort phenomenon. J Natl Cancer

Inst 1996;88:727-33

Lebwohl D, Canetta R. Clinical development of platinum

complexes in cancer therapy: An historical perspective and an

update. Eur J Cancer 1998;34:1522-34.

Taguchi T, Nazneen A, Abid MR, Razzaque MS. Cisplatinassociated

nephrotoxicity and pathological events. Contrib

Nephrol 2005;148:107-21.

Stephenson WT, Poirier SM, Rubin L, Einhorn LH. Evaluation

of reproductive capacity in germ cell tumor patients following

treatment with cisplatin, etoposide, and bleomycin. J Clin Oncol

;13:2278-80.

Zhang X, Yamamoto N, Soramoto S, Takenaka I. Cisplatin-induced

germ cell apoptosis in mouse testes. Arch Androl 2001;46:43-9.

Vawda AI. Effect of testosterone on cisplatin-induced testicular

damage. Arch Androl 1994;32:53-7.

Malarvizhi D, Mathur PP. Effect of cisplatin on testicular function

in rats. Indian J Exp Biol 1996;34:995-8.

Narayana K. Cisplatin induces duplex 3’ overhangs and 5’ blunt

ends in epididymal epithelium in a Bax-dependent manner

without any protection from L-ascorbic acid. Eur J Pharmacol

;641:238-45.

Ali BH, Al Moundhri MS. Agents ameliorating or augmenting the

nephrotoxicity of cisplatin and other platinum compounds: A reviewof some recent research. Food Chem Toxicol 2006;44:1173-83.

Mukherjee K, Tripathi A, Kulkarni KS. Evaluation of the efficacy

of ‘Speman’ in the management of male subfertility. Indian J Clin

Pract 2003;13:29-31.

Sahu KC. Clinical observations on the use of speman in the

treatment of spermatorrhoea. Med Digest 1954;9:485.

Mukherjee M, Rai DK, Dubey GP. Testicular changes in

electroshock-induced stress and effect of Speman. Probe

;29:300-6.

Mathur R, Kulshrestha R. Restitution of ceric sulphate-induced

sterility in rats by zinc and Speman. J Sci Res 1984;6:11-4.

Rathore HS. Protection of mouse testes, epididymis and adrenals

with Speman against cadmimum intoxication. Med Surg

:26:33-5.

Bhan A, Rathore H. Prevention of Hexachlorocyclohexane (HCH)-

induced changes in selected reproductive organs of mice with a

herbal drug speman in acute exposure. Indian J Occup Health

;39:1-7.

CPCSEA (Committee for the Purpose of Control and Supervision

on Experiments on Animals) CPCSEA guidelines for laboratory

animal facility. Indian J Pharmacol 2003;35:257-74.

NRC (National Research Council). Guide for the care and use

of laboratory animals. Institute of Laboratory Animal Resources

(ILAR), Commission on Life Sciences. Washington, DC: National

Academy Press; 1996. p. 125.

Mclaren A. Germ cells and germ cell transplantation. Int J Dev

Biol 1998;42:855-60.

Subbarao VV, Gupta ML, Arora HL. Effect of Speman on the testes.

Probe 1973:12:197-200.

Belsey MA, Moghissi KS, Eliasson R, Paulsen CA, Callegos AJ,

Prasad MR. Laboratory manual for the examination of human

semen and semen-cervical mucus interaction. Singapore: Press

concern. 1980. p. 10.

Talbot P, Chacon RS. A triple stain technique for evaluating

normal acrosome reaction of human sperm. J Exp Zool 1981;215:

-8.

Jeyendran RS, Van der Ven HH, Zaneveld LJ. The hypoosmotic

swelling test: An update. Arch Androl 1992;29:105-16.

Bergmayer HU. Methods of enzymatic analysis. In: Bergmayer HU,

editor. Verlag Chemie Weinheim, New York: Academic Press; 1974.

p. 447-89.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein

measurement with the Folin phenol reagent. J Biol Chem

;193:265-75.

Rao AJ, Chakraborti R, Kotagi SG, Ravindranath N. Effect of

constant infusion of gonadotropin releasing hormone (GnRH)

agonist buserelin and antagonist CDB 2085 A using osmotic

minipumps on testicular function in adult male bonnet monkey

(Macaca radiata). Andrologia 1990;22:567-73.

Bieber AM, Marcon L, Hales BF, Robaire B. Effects of

chemotherapeutic agents for testicular cancer on the male

reproductive system, spermatozoa, and fertility. J Androl

;27:189-200

Schlappack OK, Kratzik C, Schmidt W, Spona J, Schuster E.

Response of seminiferous epithelium to scattered radiation in

seminoma patients. Cancer 1988;62:1487-91.

Jayatilak PG, Pardanani DS, Dattatreya B, Sheth AR. Effect of an

indigenous drug (Speman) on accessory reproductive functions

of mice. Ind J Exp Biol 1976;14:170-3.

Ohl DA, Menge AC, Sonksen J. Penile vibratory stimulation in

spinal cord injured men: Optimized vibration parameters and

prognostic factors. Arch Phys Med Rehabil 1996;77:903-5.

Seethalakshmi L, Flores C, Kinkead T, Carboni AA, Malhotra RK,

Menon M. Effects of subchronic treatment with cis-platinum

on testicular function, fertility, progeny outcome, and progeny.

J Androl 1992;13:65-74.

Azu OO, Duru FI, Osinubi AA, Oremosu AA, Norohna CC,

Okanlawon AO. Long-term treatment with Kigelia africana fruit extract

ameliorates the testicular toxicity following cisplatin administration

in male Sprague-Dawley rats. Eur J Pharmacol 2010;641:238-45.

Schrader M, Muller M, Straub B, Miller K. Testicular sperm

extraction in azoospermic patients with gonadal germ cell tumours

prior to chemotherapy-a new therapy option. Asian J Androl

;4:9-15.

Seaman F, Sawhney E, Giammona CJ, Richburg JH. Cisplatininduced

pulse of germ cell apoptosis precedes long-term elevated

apoptotic rates in C57/BL/6 mouse testis. Apoptosis 2003;8:101-8.

Sawhney P, Giammona CJ, Meistrich ML, Richburg JH. Cisplatininduced

long-term failure of spermatogenesis in adult C57/B1/6J

mice. J Androl 2005;26:136-45.

Khaleeluddin K, Santpur SR, Rajaram P, Durgamba KK. Clinical

trials in cases of oligozoospermia with Speman. Probe 1973;4:203.

Pardanani DS, Delima RJ, Rao RV, Vaze AY, Jayatilak PG, Sheth AR.

Study of the effects of Speman on semen quality in oligospermic

men. Ind J Surg 1976;38:34.

Dupont E, Labrie F, Luu-The V, Pelletier G. Ontogeny of 3β-hydroxy

steroid dehydrogenase/Δ5-Δ4isomerase (3β-HSD) in rat testis as

studied by immunocytochemistry. Anat Embryol (Berl) 1993;187:583-9.

Nalini B, Shenoy SK, Geetha B, Anuradha, Vijay KV. The role

of herbal drugs in infertile couples. Obstet Gynaecol Today

;8:281-7.




DOI: http://dx.doi.org/10.22377/ijgp.v5i4.215

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