Ameliorative potential of Vigna mungo seeds on hyperglycemia mediated oxidative stress and hyperlipidemia in STZ diabetic rats

Jangra Meenu, Sunil Sharma, Manoj Kumar

Abstract


Ethnomedicinally the seeds of Vigna mungo are used as cooling astringent, diet during fever, poultice for abscesses, soap alternative. The increased oxidative stress plays an important role in the etiology and pathogenesis of diabetes mellitus and its various complications. This study was designed to examine the effect of hydroalcoholic extract (VME) of V. mungo seeds on STZ-diabetic rats by measuring glycemia, lipid profile, and lipid peroxidation parameters (MDA, PCO, and GSH) and antioxidant enzymes activities (SOD, CAT, and GPx). The levels of glucose, TG, TC, MDA, and PCO were increased significantly whereas the levels of serum insulin, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and Glutathione peroxidise (GPx) were decreased in STZ induced diabetic rats. Administration of VME (200 mg/kg bw and 400 mg/kg bw p.o.) to diabetic rats for 28 days showed a significant decrease
in serum glucose, TG, TC, MDA, and PCO. In addition, we also summarize here that the levels of serum insulin, superoxide dismutase, catalase, GPx, and reduced glutathione (GSH) were increased in VME treated diabetic rats. The antioxidant and antihyperglycemic effect of VME was compared with glibenclamide, a well-known antioxidant and antihyperglycemic drug. The findings in this study suggest that the VME possesses a significant favourable effect on antioxidant defense system in addition to its antidiabetic effect. Nonetheless, this study provides evidence that could help explain how the traditional use of V. mungo has been successful in the treatment of various disorders in humans.
Key words: Antihyperglycemic, oxidative stress, Vigna mungo

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Bhat, M., Zingarde S.S., Bhargava S.Y., Kumar A.R., Joshi B.N.,

Antidiabetic Indian plants: A good source of potent amylase

inhibitors. Evidence Based on Complementary and Alternative

Medicine, doi:10.1093/ecam/nen040.

World Health Organization (WHO), 2006. Diabetes Programme.

Available from: http://www.who.Int/diabetes/en/[Last accessed

on 2007 Oct 15].

Duke JA. Handbook of Legumes of World Economic Importance.

New York, London: Plenum Press; 1981.

Mazur WM, Duke JA, Wahala K, Rasku S, Adlercreutz H.

Isoflavonoids and lignans in legumes: Nutritional and health

aspects in humans. Nutr Biochem 1998;9:193-200.

Seneviratne GI, Harborne JB. Constituent flavonoids and induced

isoflavonoids as taxonomic markers in the genus Vigna. Biochem

Syst Ecol 1992;20:459-67.

Duranti M. Grain legume proteins and nutraceutical properties.

Fitoterapia 2006;77:67-82.

Harborne JB. Phytochemical Methods. London: Chapman and

Hall; 1998. p. 60.

Miller LC, Tainter ML. Estimation of ED50 and its error by means

of logarithmic probit paper. Proc Soc Exp Biol Med 1944;57:261-4.

Draper HH, Hadley M. Malondialdehyde determination as index

of lipid peroxidation. Methods Enzymol 1990;86:421-31.

Reznick AZ, Packer L. Methods in Enzymology. New York:

Academic Press; 1994. p. 357.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys

;82:70-7.

Jollow DJ, Mitchell JR, Zamppaglione N, Gillette JR. Bromobenzene

induced liver necrosis. Protective role of glutathione and evidence

for 3,4-bromobenzene oxide as the hepatotoxic metabolite.

Pharmacology 1974;11:151-69.

Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.

Beauchamp C, Fridovich I. Superoxide dismutase: Improved

assays and an assay applicable to acrylamide gel. Anal Biochem

;44:276-87.

Flohe L, Gunzler WA. Assays of glutathione peroxidase. Methods

Enzymol 1984;105:114-21.

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein

measurement with the Folin phenol reagent. J Biol Chem

;193:265-75.

Hakim ZS, Patel BK, Goyal RK. Effects of chronic ramipril

treatment in streptozotocin-induced diabetic rats. Ind J Physiol

Pharmacol 1997;41:353-60.

Rajkumar L, Srinivasan N, Balasubramanian K, Govindarajulu P.

Increased degradation of dermal collagen in diabetic rats. Ind J

Exp Biol 1991;29:1081-3.

Kajimoto Y, Kaneto H. Role of oxidative stress in pancreatic β-cell

dysfunction. Ann N YAcad Sci 2004;1011:168-76.

Yeh GY, Eisenberg DM, Kaptchuk TJ, Phillips RS. Systematic

review of herbs and dietary supplements for glycemic control in

diabetes. Diabetes Care 2003;26:1277-94.

Verges B. Lipid abnormalities in diabetes mellitus. Rev Med Interne

;12:277-80.

Merzouk H, Madani S, Chabane Sari D, Prost J, Bouchenak M,

Belleville J. Time course of changes in serum glucose, insulin, lipids

and tissue lipase activities in macrosomic offspring of rats with

streptozotocin-induced diabetes. Clin Sci (Lond) 2000;98:21-30.

Solanki YB, Jain SM. Antihyperlipidemic activity of Clitoria

ternatea and Vigna mungo in rats. Pharm Biol 2010;48:915-23.

Oztürk Y, Altan VM, Yildizoğlu-Ari N. Effect of experimental

diabetes and insulin on smooth muscle functions. Pharma

Rev1996;48:69-74.

Lery V, Zaltzber H, Ben-Amotz A, Kanter Y, Aviram M. β-Carotene

affects antioxidant status in non-insulin dependent diabetes

mellitus. Pathophysiol 1999;6:157-62.

Szkudelski T. The mechanism of alloxan and streptozotocin action

of β-cells of the rat pancreas. Physiol Res 2001;50:537-46.

Maritim AC, Sanders RA, Watkins JB 3rd. Diabetes, oxidative

stress, and antioxidants: A review. J Biochem Mol Toxicol

;17:24-38.

Ohkuwa T, Sato Y, Naoi M. Hydroxyl radical formation in diabetic

rats induced by streptozotocin. Life Sci 1995;56:1789-98.

Winterbourn CC. Concerted antioxidant activity of glutathione

and superoxide dismutase. In: Packer L, Fuchs J, editors. Biothiols

in Health and Disease. New York: Marcel Dekker Inc; 1995.

p. 117-34.

Loven D, Schedl H, Wilson H, Daabees TT, Stegink LD, Diekus M,

et al. Effect of insulin and oral glutathione on glutathione levels

and superoxide dismutase activities in organs of rats with

streptozotocin induced diabetes. Diabetes 1986;35:503-7.

Coskun O, Kanter M, Korkmaz A, Oter S. Quercetin, a flavonoid

antioxidant, prevents and protects streptozotocin-induced

oxidative stress and beta-cell damage in rat pancreas. Pharmacol

Res 2005;51:117-23.

Meghana K, Sanjeev G, Ramesh B. Curcumin prevents

Streptozotocin induced islet damage by scavenging free radicals:

a prophylactic and protective role. Eur J Pharmacol 2007;577:

-91.

Qi, XY, Chen WJ, Zhang LQ, Xie BJ. Mogrosides extract from

Siraitia grosvenori scavenges free radicals in vitro and lowers

oxidative stress, serum glucose, and lipid levels in alloxan-induced

diabetic mice. Nutr Res 2008;28:278-84.

Bagri P, Ali M, Aeri V, Bhowmik M, Sultana S. Antidiabetic effect

of Punica granatum flowers: Effect on hyperlipidemia, pancreatic

cells lipid peroxidation and antioxidant enzymes in experimental

diabetes. Food Chem Toxicol 2009;47:50-4.

Raha S, Robinson BH. Mitochondria, oxygen free radicals, disease

and ageing. Trends Biochem Sci 2000;25:502-8.

Ravi K, Ramachandran B, Subramanian S. Protective effect

of Eugenia jambolaa seed kernel on tissue antioxidants

in streptozotocin-induced diabetic rats. Biol Pharm Bull

;27:1212-7.

Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect

of Aloe vera gel extract in streptozotocin-induced diabetic rats.

Pharmacol Rep 2005;57:90-6.

Farombi EO, Ige OO. Hypolipidemic and antioxidant effects of

ethanolic extract from dried calyx of Hibiscus sabdariffa in alloxaninduced

diabetic rats. Fundam Clin Pharmacol 2007;21:601-9.

Zhang XF, Tan BK. Antihyperglycemic and antioxidant properties

of Andrographis paniculata in normal and diabetic rats. Clin Exp

Pharmacol Physiol 2000;27:358-63.

Santini SA, Marra G, Giardina B, Cotroneo P, Mordente A,

Martorana GE, et al. Defective plasma antioxidant defenses and

enhanced susceptibility to lipid peroxidation in uncomplicated

IDDM. Diabetes 1997;46:1853-8.

El-Missiry MA, El Gindy AM. Amelioration of alloxan induced

diabetes mellitus and oxidative stress in rats by oil of Eruca sativa

seeds. Ann Nutr Metab 2000;44:97-100.

Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin

in streptozocin-induced diabetic rats. Comp Biochem Physiol C

Toxicol Pharmacol 2003;135C:357-64.




DOI: http://dx.doi.org/10.22377/ijgp.v5i4.211

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