Beneficial effects of carrot pectin against lead intoxication in Wistar rats

Ouardia Ouldali, A. Aoues, B. Meddah, M. Slimani, A. Nicolas, O. Kharoubi


The aim of the present study was to investigate the beneficial action, in vivo, of pectin against subacute lead acetate (350 mg/l) intoxication. The adverse effects of lead on the haematological disturbances that concerned, more precisely, the decrease of red blood corpuscle life duration and on the appearance of ever granulated basophilic haematites by inhibiting an enzyme responsible for haeme synthesis have been demonstrated after 1 month of oral lead administration to female Wistar rats. Also, this caused an elevation of the blood lead level as compared with the control group. The introduction of carrot pectin to a level of 3% in the feeding
of intoxicated rats has shown a chelating and correcting effect on haematological disturbances caused by lead toxicity, which is
reflected by a significant decrease (P<0.05) of blood lead (from 117 to 65 to 19 μg/l), zinc protoporphyrine (portophyrine-zinc from 7.7 to 5.1 to 3.5 μg/g of Hb), increase in haemoglobin to 27% (from 5.09 to 6.05 to 7.79%) and iron to 8% (from 1.34 to 0.9 to 0.5%) of the treated rats by pectin as compared with the untreated groups. Differences in blood lead were significant between the control diet and the addition of pectin therefore suggesting that pectin fibre ingestion in diets decreases the risk of lead poisoning.
Key words: Blood lead, carrot pectin, haemoglobin, lead acetate, ZPP

Full Text:



Ragan P, Turner T. Working to prevent lead poisoning in children:

Getting the lead out. JAAPA 2009;22:40-5.

Pokras MA, Kneeland MR. Lead poisoning: Using transdisciplinary

approaches to solve an ancient problem. EcoHealth 2008;5:379-85.

Mañay N, Cousillas AZ, Alvarez C, Heller T. Lead contamination

in Uruguay: the "La Teja" neighborhood case. Rev Environ Contam

Toxicol 2008;195:93-115.

Rossi E. Low level environmental lead exposure -a continuing

challenge. Clin Biochem Rev 2008;29:63-70.

Meyer P, Brown M, Falk H. Global approach to reducing lead

exposure and poisoning. Mutat Res 2008;659:166-75.

Alleman H, Cosendey B, Lob M. Lead poisoning by cutaneous

drug absorption. Swiss Med Wkly 1986;116:888-91.

Pearce JM. Burton's line in lead poisoning. Eur Neurol


Tomkuni K Ichiba M. Effect of lead on the activity of erythrocyte

porphobilinogen desaminase in vivo and in vitro. Toxicol Lett


Testud F. Pathology in toxic workplace ESKA the cassague. 2nd ed.

p. Part 4: metals; lead, thallium, vanadium, zinc, 1998. p. 169-78.

Zhang ZW, Moon CS, Watanabe T, Shimbo S, He FS, Wu YQ, et al.

Background exposure of urban populations to lead and cadmium:

comparison between China and Japan. Int Arch Occup Environ

Health 1997;69:273-81.

Lightfoot TL, Yeager JM. Pet bird toxicity and related environmental

concerns. Vet Clin North Am Exot Anim Pract 2008;11:229-59, vi.

Martell E, Hancock RD. Metal Complexes in Aqueous Solutions.

Modern Inorganic Chemistry. New York: Plenum Press; 1996.

El-Zoghbi M, Sitohy MZ. Mineral absorption by albino rats as

affected by some types of dietary pectins with different degrees

of esterification. Nahrung 2001;45:114-7.

Le Gall B, Tarrana F, Renault D, Wilk JC, Ansoborlo E. Comparison

of Prussian blue and apple-pectin efficacy on 137Cs decorporation

in rats. Biochimie 2006;88:1837-41.

Degtiareva TD, Katsnel'son BA, Pirvalova LI, Beresnova Olu,

Gurvich VB, Kuz'min SV, et al. Use of biologically active substances

in preventing the toxic action of some heavy metals. Gig Sanit


Naudin O, Josselin P, Baynast RD. Process for the production of

food fibres from carrots and fibres contained by this process. 1992.

Application Number: EP19910402240.

Albahary C, Akhgren C. In vivo determination of lead in the

skeleton after occupational exposure to lead. British Journal of

Industrial Medicine 1980;37:109-113.

Joselow MM, Flores J. Application of the zinc protoporphyrin (ZP)

test as a monitor of occupational exposure to lead. Am Ind Hyg

Assoc J 1977;38:63-6.

Dorward A, Yagminas AP. Activity of erythrocytes deltaaminolevulinic

acid dehydratase in the female monkey Macaca

fasciularis: "Kinettes” year analysis in control and lead-exposed

animals' comp. Comp Biochem Physiol Biochem Mol Biol


Paglia DE, Valentine WN, Dahlgren JG. Effects of low-level

exposure on pyrimidine S-nucleotidase enzymes and others. J

Clin Invest 1985;56:1194-9.

Trakhtenberg IM, Lukovenko VP, Korolenko TK, Ostroukhov

VA, Demchenko PI, Rabotyaga TY, et al. Preventive use of

pectin for chronic exposure to lead in production. Lik Sprava


Gibson KD, Neuberger A, Scott JJ. The purification and properties

of delta-aminolevulinic acid dehydratase. Biochem J 1995;


Cezard C, Hagnenoer JM. Toxicology of lead in humans. In:

Lavoisier, editor. Paris: Tec § Doc; 1994.

Tahiri M, Doco T, Tressol JC, Pellerin P, Pepin D, Rayssiguier Y,

et al. The Rhamnogalacturonan II, a pectin polysaccharide,

decreases intestinal absorption and tissue retention of lead

in rats. Bulletin de l’Office International de la Vigne et du vin




  • There are currently no refbacks.