Antimicrobial and wound healing activities of leaves of Alternanthera sessilis Linn

Sunil S. Jalalpure, Nitin Agrawal, M.B. Patil, R. Chimkode, Ashish Tripathi

Abstract


Th e leaves of Alternanthera sessilis (Linn.) R.Br.ex DC (Family Amaranthaceae) were exhaustively extracted by soxhlet apparatus withdiff erent solvents like, petroleum ether (40-60ºC), chloroform, acetone, methanol and distilled water in ascending order of the polarity. All the fi ve extracts were subjected to antimicrobial screening by using the cup plate and turbidimetric methods. Th e chloroform extract among all fi ve extracts showed maximum zone of inhibition and signifi cant MIC values in above two methods respectively. Hence chloroform extract was taken for the screening of wound healing activity and models adopted for wound healing activity were, incision, excision and granuloma studies. Th e chloroform extract of leaves of Alternanthera sessilis at a dose of 200 μg/mL (orally) in all models showed signifi cant results. Contraction of wound area (P < 0.005) at 16th day, time for complete epithelization in 16 days (P < 0.0001), and scar area after complete epithelization was found to be 33.2 ± 0.0730 (P < 0.0008) in excision wound model, wound breaking strength 388 ± 5.85 grams (P < 0.0001) in incision wound model, granuloma dry weight 47.7 ± 2.29, granuloma breaking  strength 247 ± 10.2 (P < 0.022) in granuloma studies. All the results were signifi cant for diff erent parameters in wound healing activity when compared with control group. Presence of sterols in chloroform extract was also confi rmed by preliminary phytochemical investigation, TLC and HPTLC methods.
Key words: Alternanthera sessilis, antimicrobial, chloroform extract, wound healing

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DOI: http://dx.doi.org/10.22377/ijgp.v2i3.18

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