Antibacterial activity and qualitative phytochemical analysis of Vitex mollis fruit

Francisco Delgado-Vargas, Fernando Félix-Favela, Juan F. Pio-León, Gabriela López-Angulo, José A. Lopez-Valenzuela, Sylvia P. Díaz-Camacho, Magdalena de-J. Uribe-Beltrán

Abstract


The pulp of the Vitex mollis fruit is edible and traditionally used to treat diarrhoea. The antibacterial activity of this fruit is reported here for the first time. The fruit pulp was extracted with methanol (ME) and the extract was fractionated with solvents. ME and their fractions [hexanic (HF), chloroformic (CF), ethyl acetate (EAF) and aqueous (AqF)] were assayed against human pathogenic bacteria (microdilution test) and their phytochemicals determined (qualitative chemical determinations). The samples (i.e., ME, HE, CF, EAF and AqF) showed antibacterial activity; EAF was the most active, showing such activity against Shigella dysenteriae [minimal inhibitory concentration (MIC)=2 mg/ml]. Phenolics were mainly found in ME and EAF; compounds of this chemical family are well known for their antidiarrhoeal and antimicrobial activities. The reported antibacterial activity and phenolics content of V. mollis fruit could be associated with its use in the treatment of diarrhoea.
Key words: Antibacterial activity, diarrhoea, phytochemical analysis, Vitex mollis

Full Text:

PDF

References


Schmidt FR. The challenge of multidrug resistance: Actual

strategies in the development of novel antibacterials [MINIREVIEW].

Appl Microbiol Biotechnol 2003;63:335-43.

Boletín Epidemiología. México, D.F.: Dirección General de

Epidemiología. Secretaría de Salud [last updated on 2009 Aug 5]

Available from: http://www.dgepi.salud.gob.mx/boletin/boletin.

htm [last cited on 2009 Sep 3].

Fowler MW. Plants, medicines and man [Review]. J Sci Food Agric

;86:1797-804.

Palombo EA. Phytochemicals from traditional medicinal plants

used in the treatment of diarrhoea: Modes of action and effects

on intestinal function. Phytother Res 2006;20:717-24.

Pennington TD, Sarukhán J. Tropical trees from Mexico. Manual

for identification of the main species from Mexico: UNAM FCE;

Montiel-Herrera M, Camacho-Hernandez IL, Rios-Morgan

A, Delgado-Vargas F. Partial physicochemical and nutritional

characterization of the fruit of Vitex mollis (Verbenaceae). J Food

Comp Anal. 2004;17:205-15.

Moldenke HN. Additional notes on the genus Vitex. XVII.

Phytologia 1980;46:42.

Osuna L, Tapia-Perez ME, Jiménez-Ferrer JE, Carrillo-Quiróz

BA, Silva-Sanchez J. Screening of Alternanthera repens, Boerhavia

coccinea, Flaveria trinervia, Tournefortia densiflora, and Vitex mollis

extracts to evaluate their antibacterial activity and effect on smooth

muscle. I. Pharm Biol 2005;43:749-53.

Suleiman MM, Yusuf S. Antidiarrheal activity of the fruits of Vitex

doniana in laboratory animals. Pharm Biol 2008;46:387-92.

Wall ME, Wani MC, Brown DM, Fullas F, Oswaldo JB, Josephson

FF, et al. Effects of tannins on screening of plants of plants

extracts for enzyme inhibitory and techniques for their removal.

Phytomedicine 1996;3:281-5.

Lennette EH, Balows A, Hausler WL, Shadomy HJ. Susceptibility

tests: Microdilution and macrodilution in broth. In: Lennette

EH, Balows A, Hausler WL, Shadomy HJ, editors. Manual of

Clinical Microbiology, Buenos Aires. Argentina: Editorial Medica

Panamericana S.A.; 1987.

Cos P, Vlietinck AJ, Berghe DV, Maes L. Anti-infective potential

of natural products: How to develop a stronger in vitro 'proof-ofconcept'.

J Ethnopharmacol 2006;106:290-302.

Domínguez AX. Métodos de investigación fitoquímica. 4th ed.

México, D.F.: Limusa; 1988.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents

of some Nigerian medicinal. Afr J Biotechnol 2005;4:685-8.

Harborne JB. Phytochemical Methods (a Guide to Modern

Techniques of Plant Analysis). London, UK: Chapman and Hall

Ltd; 1980.

Hernandez MM, Heraso C, Villarreal ML, Vargas-Arispuro I,

Aranda E. Biological activities of crude plant extracts from Vitex

trifolia L. (Verbenaceae). J Ethnopharmacol 1999;67:37-44.

Kilani AM. Antibacterial assessment of whole stem bark of

Vitex doniana against some enterobactriaceae. Afr J Biotechnol

;5:958-9.

Nyiligira E, Viljoen AM, Van Heerden FR, Van Zyl RL, Van Vuuren

SF, Steenkamp PA. Phytochemistry and in vitro pharmacological

activities of South African Vitex (Verbenaceae) species. J

Ethnopharmacol 2008;119:680-5.

Rios JL, Recio MC. Medicinal plants and antimicrobial activity. J

Ethnopharmacol 2005;100:80-4.




DOI: http://dx.doi.org/10.22377/ijgp.v4i4.162

Refbacks

  • There are currently no refbacks.