A report on the antioxidant activity of the powder of the entire plant of Phyllanthus reticulatus Poir

V. Maruthappan, K. Sakthi Shree


In the present study, we determined the antioxidant activity of the entire plant of Phyllanthus reticulatus by performing different
in vitro antioxidant assays, including 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) radical scavenging, β-carotene bleaching, superoxide anion radical scavenging, reducing power and metal chelating assay at different concentrations (100, 200 and 400 μg/ml). The entire plant powder of P. reticulatus shows good antioxidant activity of about 90.0% when compared with standard Butylated Hydroxy Toluene (BHT) (85%) at a concentration of 400 μg/ml. Results obtained reveal that methanolic extracts of entire plant of P. reticulatus possess higher antioxidant activity when compared with ethanolic extracts. Thus, this study suggests that P. reticulatus plant can be
used as a potent source of natural antioxidants.
Key words: Antioxidant activity, DPPH, free radical, Phyllanthus reticulatus

Full Text:



Hertog MG, Feskens EJ. Dietary antioxidant flavonoids and risk

of coronary heart disease: The Zutpher Elderly study. Lancet


Wilson RL. Free radicals and tissue damage, mechanistic evidence

from radiation studies. Biochemical Mechanisms of Liver Injury.

New York: Academic Press; 1988. p. 123.

Cai YZ, Sun M. Antioxidant activity of betalins from plants of the

Amaranthacea. J Agric Food Chem 2003;131:2837-42.

Rice-Evans C, Miller N. Antioxidant properties of phenolic

compounds. Trends Plant Sci 1997;2:152-9.

Gopalakrishna KB. Flora of Udupi. 1st ed. Udupi: Indian Naturalist;

p. 578.

Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Vol. 3.

Dehradun: Bishen Singh and Mahendra Pal Singh; 1991. p. 2219-20.

Yoshida T, Seno K, Takama Y, Okanda T. Tannins and related

polyphenol of Euphorbiaceous plants. Phytochemistry


Nandkarni KM. Indian Materia Medica. Indian Maretia Med


Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants

– a compendium of 500 species. Vol. 4. Madras: Orient Longman

Ltd; 1995; p. 264-5.

Council of Scientific and Industrial Research. The Wealth of

India, 2005. Vol. 7. New Delhi: National Institute of Science

Communication and Information Resources; 2003. p. 34.

Dhanabal SP, Sureshkumar M. Hypoglycemic effect of ethanolic

extract of Musa sapientum on alloxan induced diabetes mellitus

in rats and its relation with antioxidant potential. J Herbal

Pharmacother 2005;5:7-19.

Mensor LL, Menezes FS. Screening of Brazilian plant extracts

for antioxidant activity by the use of DPPH free radical method.

Phytother Res 2001;15:127-30.

Jayasri MA, Mathew L, Radha A. A report on the antioxidant

activity of leaves and rhizomes of Costus pictus D. Don Int J Integ

Biol 2009;5:20-6.

Miller HE. A simplified method for the evaluation of antioxidant.

J Am Oil Chem Soc 1971;48:91-5.

Yen GC, Chen HY. Antioxidant activity of various tea extracts in

relation to their antimutagenicity. J Agric Food Chem 1995;43:27-32.

Dinis TC, Madeira VM. Action of phenolic derivatives

(acetaminophen, salicylate and 5-aminosalicylate) as inhibitors

of membrane lipid peroxidation and as peroxyl radical scavengers.

Arch Biochem Biophys 1994;36:161-9.

Nishikimi M, Rao NA. The occurrence of superoxide anion in

the reaction of reduced phenazine methosulphate and molecular

oxygen. Biochem Biophys Res Commun 1972;48:849-53.

Folin O, Ciocalteu V. On tyrosine and tryptophan determination

in proteins. J Biol Chem 1927;27:627-50.

Amarowicz R, Pegg PB. Free-radical scavenging capacity and

antioxidant activity of selected plant species from the Canadian

prairies. Food Chem 2004;84:551-62.

Gülçin I, Elias R, Gepdiremen A, Chea A, Topal F. Antioxidant

activity of bisbenzylisoquinoline alkaloids from Stephania rotunda:

Cepharanthine and fancchinoline. J Enzyme Inhib Med Chem


Aswatha Ram HN, Shreedhara CS, Gajera FP, Zanwar SB.

Antioxidant studies of aqueous extract of Phyllanthus reticulatus

Poir. Pharmacol 2008;1:351-64.

Jun MT, Ujianzhang L. Identification and evaluation of antioxidant

activities of bamboo extracts. For Stud China 2004;6:1-5.

Jayaprakasha GK, Singh RP. Antioxidant activity of grape seed

(Vitis vinifera) extracts on peroxidation models in vitro. Food Chem


Suja KP, Jayalekshmy A. Antioxidant activity of sesame cake

extract. Food Chem 2007;91:213-9.

Singh RP, Murthy KN. Studies on the antioxidant activity of

Pomegranate (Punica granatum) peel and seed extracts using

in vitro models. J Agri Food Chem 2002;50:81-6.

Meir S, Kanner J. Determination and involvement of aqueous

reducing compounds in oxidative defense systems of various

senescing leaves. J Agric Food Chem 1995,43:1813-919.

Yildirim A, Mavi A. Comparison of antioxidant and antimicrobial

activities of Tilia (Tilia argentea Desf Ex DC), Sage (Salvia triloba

L.) and Black Tea (Camellia sinensis) extract. J Agric Food Chem


Zou YP, Lu YH. Antioxidant activity of a flavonoids rich extract of

Hypericum perforatum L. in vitro. J Agric Food Chem 2004;52:5032-9.

Hsu CL, Chen W. Chemical composition, physical properties and

antioxidant activities of yam flours as affected by different drying

methods. Food Chem 2003;83:85-92.

Elmastas M, Gulcin I. Radical scavenging activity and antioxidant

capacity of bay leaf extracts. J Iran Chem Soc 2006;3:258-66.

Kanatt RC. Antioxidant potential of mint (Mentha spicata L.) in

radiation processed lamb meat. Food Chem 2007;100:451-8.

Halliwell B, Gutteridge JM. Free Radicals Biol Med. Oxford:

Clarendon Press; 1989. p. 23-30.

Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000;63:1035-42.

Sakihama Y, Cohen MF. Plant phenolic antioxidant and prooxidant

activities phenolics-induced oxidative damage mediated by metals

in plants. Toxicology 2002;177:67-80.

DOI: https://doi.org/10.22377/ijgp.v4i4.158


  • There are currently no refbacks.