Quantitative estimation of silybin in Silybum marianum mother tincture using high performance liquid chromatography

Purnima D. Hamrapurkar, Leena V. Thakurdesai, Mitesh D. Phale


Silybin is a characterising compound present in the plant Silybum marianum, used as an hepatoprotective. A simple, accurate and
sensitive HPLC method was developed for estimation of silybin. The chromatographic separation was carried out on RP C18 (Waters)
column (250 × 4.6 mm, 10 μm) using mobile phase, phosphate buffer: water (65:35 % v/v) pumped at a flow rate of 1 ml/min. The
eluent was monitored at 288 nm. The method is specific and linear over the range of 10-200 ng/ml. The method was statistically
validated for precision, accuracy, robustness and recovery. The HPLC method can be applied for identification and on of silybin in
herbal extracts of Silybum marianum.
Key words: HPLC, mother tincture, quantitative estimation, silybin, Silybum marianum

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Premila MS. Emerging frontiers in the area of hepatoprotective

herbal drugs. Indian J Nat Prod 1995;11:3-12.

Homeopathic Pharmacopoeia of India, vol. 1, Government of India,

Ministry of Health and family welfare, controller of publication.

New Delhi: 1971. p. 7,95.

Khare CP. Indian Medicinal Plants. India: Springer; p. 426.

Morazzoni P, Bombardelli E. Silybum marianum (Carduus

marianus). Fitoterapia 1995;66:3-42.

Flora K, Hahn M, Rosen H, Benner K. Milk Thistle (Silybum

marianum) for the therapy of liver disease. Am J Gastroenterol


Tuchweber B, Trost W, Salas M, Sieck R. Prevention of

praseodymium-induced hepatotoxicity by silybin. Toxicol Appl

Pharmacol 1976;38:559-70.

Sonnenbichler J. Zetl I. Biochemistry of a liver drug from the thistle

Silybum marianum. Planta Medica 1992;58:A580-1.

Kirtikar KR, Basu BD. Indian Medicinal Plant. Vol. 2. Dehradun:

Springer; 1995. p. 1417-8.

Wagner H, Blandt S. Plant Drug Analysis. 2nd ed. Dehradun:

Springer; 1996. p. 234.

Gocan S, Cimpman G, Muresan L. Automated multiple

development thin layer chromatography of some plant extracts. J

Pharm Biomed Anal 1996;14:1221-7.

Rajasekaran A. A HPTLC method for analysis of flavonolignan

content in extract. Eastern Pharmacist 1997;40:131-2.

Weyhenmeyer R, Rickling B, Hans B, Kramarczyk R, Krumbiegel

G. Two high-performance liquid chromatographic assays for

the determination of free and total silibinin diastereomers in

plasma using column switching with electrochemical detection

and reversed-phase chromatography with ultraviolet detection. J

Chromat B 1995;670:267-77.

Tittel G, Wagner H. Hochleistungsflussigchromatographische

trennung von silymarinen und deren bestimmung im rohextrakt

von Silybum marianum gaertn. J Chromat A 1977;135:499-501.

Ding T, Tian S, Zhang Z, Gu D, Chen Y, Shi Y, et al. Determination

of active component in silymarin by RP-LC and LC/MS. J Pharm

Biomed Anal 2001;26:156-201.

Tittel G, Wagner H. Hochleistungsflussigchromatogrphie von

silymarin: II: Quantitative bestimmung von silymarin aus Silybum

marianum durch hochleistungsflussigchromatographie. J Chromat

A 1978;153:227-32.

Quaglia MG, Bossu E, Donati E, Mazzanti G, Brandt A.

Determination of silymarin in the extract from the dried Silybum

marianum fruits by high performance liquid chromatography and

capillary electrophoresis. J Pharm Biomed Anal 1999;19:435-42.

DOI: https://doi.org/10.22377/ijgp.v4i4.153


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