Antioxidant and antimicrobial activities of isolated constituents from the bark of Polyalthia longifolia

Sankhadip Bose, Vivek V. Byahatti, Marina D Souza, Arti Bose


The bark of Polyalthia longifolia is traditionally reputed to lower blood pressure, stimulate respiration and help in fever and skin
diseases, diabetes, hypertension and vitiated conditions of vata and pitta. Preliminary phytochemical investigation of various extracts of the bark of Polyalthia longifolia showed the presence of flavonoids, alkaloids, steroids and carbohydrates. An attempt has been made to isolate flavonoids and perform antioxidant and antimicrobial activity of the same. We carried out DPPH radical scavenging assay, nitric oxide scavenging assay, metal chelating activity and reducing power activity in antioxidant activity. In antimicrobial activity, we used six microorganisms, which included two Gram positive, two Gram negative bacteria and two fungi. Both the isolated flavonoids exhibited a concentration-dependant free radical scavenging capacity. Isolated compounds showed promising results against various
micro-organisms in comparison with standard drugs (Penicillin, Gentamicin and Ketoconazol).
Key words: Antioxidant, antimicrobial, isolated compounds

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Dev S. Ethno botanical survey of traditional plants. Curr Sci1997;73;909-28.

Yoganarsimhan S.N. Medicinal Plants of India-Karnataka.

Bangalore, India: Interline Publishing (P).Ltd; 2000.

Kirtikar KR, Basu BD. Indian Medicinal Plants. Vol 1, Dehradun:

Industrial Books Distributer; 1995.

Phadnis AP, Patwardhan SA, Dhaneshwar NN, Tavle SG.

Clerodane diterpenoids from Polyalthia longifolia. Phytochemistry


Chakrabarty M. 6,7, Dimethoxyonychine and other alkaloids of

Polyalthia longifolia. Indian J Chem Br 1990;29:394-5.

Wu YC, Duh CY, Wang SK, Chen KS, Yang TH. Two new natural

azafluorene alkaoids and a cytotoxic aporphine alkaloid from

Polyalthia longifolia. J Nat Prod 1990;53:1327-32.

Rashid MA, Hossain MA, Hassan CM, Reza MS. Antimicrobial

diterpenes from Polyalthia longifolia var. pendula (Annonaceae)

Phytother Res 1996;10:79-81.

Sebsibe-Ameha A, Mathur MM. Chemical composition of Asoka

(Polyalthia longifolia) tree leaves. Indian J Anim Nutr 2000;17:254-5.

Males Z, Plazibat M, Vundać VB, Zuntar I. Qualitative and

quantitative analysis of flavonoids of the strawberry tree- Arbutus

unedo L. (Ericaceae). Acta Pharm 2006;56:245-50.

Yen GC, Chen HY. Antioxidant activity of various tea extracts in

relation to their antimutagenecity. J Agric Food Chem 1995;43:27-37.

Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide

– scavenging properties of Ginkgo biloba extract EGB 761. Biochem

Biophys Res Commun 1994;201:748-55.

Dinis TC, Maderia VM, Almeida LM. Action of phenolic

derivatives (acetoaminophen, salycilate and 5-aminosalycilate) as

inhibitors of membrane lipid peroxidation and as peroxyl radical

scavengers. Arch Biochem Biophys 1994;315:161-9.

Oyaizu M. Studies on products of browning reactions:

Antioxidative activities of products of browning reaction prepared

from glucosamine. Jpn J Nutr 1986;44:307-15.

Mathew J. Antimicrobial activity of selected aromatic plants. Indian

Drugs 2005;42:28-33.

Kumari T GR, Paul PT, Ayyub S, Iyenger MA, Rao PS. Antimicrobial

activity of the essential oil of Elettaria cardamomum Maton. Indian

Drugs 2004;41:622-3.

Kuppuswamy A., Shanmuga P, Veena A. Antifungal activity of

newly sysnthesized piperidin-4-oxime derivatives. Indian Drugs


Talas-Ogras T, Ipekci Z, Bajrovic K, Goziikirmizi N. Antibacterial

activity of seed proteins of Robinia pseudoacacia. Fitoterapia


Shasrty CS, Aravind MB, Joshi SD, Bheemachari AK. Antibacterial

and antifungal activity of Thespesia populnea (L). Indian Drugs


Merith K. Antimicrobial activity of fresh plant juice on the growth

of bacteria and yeasts. J Qafqaz Univ 1997;1:27-33.

Mimica-Dukić N, Bozin B, Soković M, Mihajlović B, Matavulj M.

Antimicrobial and antioxidant activities of three mentha species

essential oils. Planta Med 2003;69:413-9.



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